Document 0559
 DOCN  M9480559
 TI    Luffin-S--a small novel ribosome-inactivating protein from Luffa
       cylindrica. Characterization and mechanism studies.
 DT    9410
 AU    Gao W; Ling J; Zhong X; Liu W; Zhang R; Yang H; Cao H; Zhang Z; Shanghai
       Institute of Biochemistry, Academia Sinica, P.R. China.
 SO    FEBS Lett. 1994 Jun 27;347(2-3):257-60. Unique Identifier : AIDSLINE
       MED/94307395
 AB    We purified and characterized a novel RIP (ribosome inactivating
       protein), Luffin-S from the seeds of Luffa cylindrica. Different from
       Luffin-A and B, which are RNA N-glycosidases with molecular weights of
       27 and 28 kDa, respectively, Luffin-S has an M.W. of only approx. 10
       kDa, much smaller than any other RIPs so far investigated. Its abundant
       resources, toxicity similar to TCS in a cell-free protein synthesis
       system and unique mechanism as phosphodiesterase, like alpha-sarcin,
       promisingly make it a potential toxic moiety of immunotoxin.
 DE    Amino Acids/ANALYSIS  Animal  Cell-Free System  Comparative Study  Drug
       Stability  Electrophoresis, Polyacrylamide Gel  Fungal
       Proteins/ANALYSIS/METABOLISM  Heat  HIV-1/DRUG EFFECTS  Molecular Weight
       Phosphoric Diester Hydrolases/*ISOLATION & PURIF/METABOLISM/
       PHARMACOLOGY  Plant Proteins/*ISOLATION & PURIF/METABOLISM/PHARMACOLOGY
       Protein Synthesis Inhibitors  Rabbits  Ribonuclease,
       Pancreatic/ANALYSIS/METABOLISM  Ribosomes/*DRUG EFFECTS  RNA,
       Ribosomal/METABOLISM  Seeds/*CHEMISTRY  JOURNAL ARTICLE

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